(Download) "Section V: Biomedical Sciences" by Georgia Journal of Science # Book PDF Kindle ePub Free
eBook details
- Title: Section V: Biomedical Sciences
- Author : Georgia Journal of Science
- Release Date : January 22, 2009
- Genre: Engineering,Books,Professional & Technical,
- Pages : * pages
- Size : 51 KB
Description
2:00 PHYTOSTEROL SUPPLEMENTATION **, Lucky Nwankwo (1), Kereen Gordon (2), Victoria Miles (2), Godwin Ifere (2), Qing He (3), Eno Ekong (3), Francis Eko (3), Joseph Igietseme (4) and Godwin Ananaba (1/2), (1) Center for Cancer Research & Therapeutic Development, (2) Clark Atlanta University, (3) Morehouse School of Medicine and (4) Centers for Disease Control and Prevention, Atlanta, GA. Interleukin-10 (IL-10) cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of The cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. IL-10 is an anti-inflammatory cytokine that depends on the JAK-STAT signaling pathway. Cancerous cells increase their production of TGF-[beta] and IL-10, which also act on surrounding cells to suppress immune response. We examined the mechanisms by which plant sterols such as [beta]-cyclodextrin ([beta]-CD) induce production of factors that can suppress or enhance immune response against prostate cancer. We hypothesize that phytosterol treatment enhances immunostimulatory effects of promonocytic THP1 cells on prostate cancer cells. In this study THPl cells were used to determine the effects of IL-10 on JAK-STAT and TGF [beta]signaling pathways. A combination of techniques such as ELISA, PCR, and Western blotting were used to determine the effects of [beta]-CD delivered phytosterols on THPl maturity and activation of immunity against prostate cancer. We found that prostate cancer cells continuously produce IL-10, a Th2 cytokine, and TARC, an angiogenic Th2 chemokine. Moreover phytosterols activate macrophages and enhance immune response. Our results suggest that phytosterols can be used as immunotherapeutic agents against prostate cancer.